Journal: Materials

Article Title: Zinc and Copper Oxide Nanoparticles: Pioneering Antibacterial and Antibiofilm Strategies for Environmental Restoration against Antibiotic-Resistant Bacteria

doi: 10.3390/ma17143444

Figure Lengend Snippet: PCR primers for16S rRNA amplification.

Article Snippet: PCR primers targeting the 16S rRNA gene were acquired from VBC-Biotech Eurofins Genomics, Vienna, Austria.

Techniques: Amplification

Journal: PLoS ONE

Article Title: Molecular Diagnostic Tools for Detection and Differentiation of Phytoplasmas Based on Chaperonin-60 Reveal Differences in Host Plant Infection Patterns

doi: 10.1371/journal.pone.0116039

Figure Lengend Snippet: Results are shown for a 4-plex assay (format used for analysis of 192 B. napus DNA extracts) on plasmid DNA controls (10 7 copies/PCR) and on genomic DNA extracted from various infected plant tissues. Beads with a positive hybridization signal in each sample are identified (*). Samples from infected plant tissues are those described in (onion, item #25; flax, item #41; vinca1, item #33; vinca2, item #35; carrot, item #15). Abbreviations: MFI, median fluorescence intensity; BN, Bois Noir; ESFY, European Stone Fruit Yellows; AY, Aster Yellows.

Article Snippet: Samples were analyzed using 16S rRNA gene-targeted universal bacterial PCR primers to ensure that amplifiable bacterial DNA was present in the extract at the dilutions analyzed. cpn 60 UT amplicons were generated under the following conditions: 1×PCR buffer (Life Technologies); 2.5 mM MgCl 2 ; 0.4 µM each primer ( ); 0.5 mM each dNTP; and 1 U Taq DNA polymerase (Life Technologies).

Techniques: Plex Assay, Plasmid Preparation, Infection, Hybridization, Fluorescence